Possible reason:Sections are dried before dyeing is conducted or the reagent is dropped

Solution:Protect the sections from drying

Possible reason:Incomplete deparaffinating

Solution:Prolong the deparaffinating time or replace xylene or alcohol

Possible reason:Sodium azide in the buffer can inhibit the activity of enzyme, resulting in dyeing failures

Solution:Use buffer without sodium azide

Possible reason:Improper incubation

Solution:Ensure the best incubation time and incubation temperature